iFISH Probe Design (
ifpd) is a Python3 package containing tools for selection of complementary oligonucleotides to build iFISH probes.
It also includes a web interface, which simplifies the procedure by removing any requirement for programming skills.
The tools allow to select sets of oligomers as iFISH probes, starting from databases of oligonucleotides complementary to specific genomic loci.
Gelali, E., Girelli, G., Matsumoto, M., Wernersson, E., Custodio, J., Mota, A., … & Bienko, M. (2019). iFISH is a publicly available resource enabling versatile DNA FISH to study genome architecture. Nature communications, 10(1), 1-15. (link)